Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Leptin Receptor (extracellular) Blocking Peptide (#BLP-LR039) is the original antigen used for immunization during Anti-Leptin Receptor (extracellular) Antibody (#ALR-039) generation. The blocking peptide binds and ‘blocks’ Anti-Leptin Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), rat dorsal root ganglion lysate (lanes 2 and 5) and mouse brain membranes (lanes 3 and 6):1-3. Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:200).
4-6. Anti-Leptin Receptor (extracellular) Antibody, preincubated with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039).
Western blot analysis of rat liver lysates:1. Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:200).
2. Anti-Leptin Receptor (extracellular) Antibody, preincubated with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039).
Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and mouse TK-1 T-cell lymphoma cell line lysate (lanes 2 and 4):1-2. Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:200).
3-4. Anti-Leptin Receptor (extracellular) Antibody, preincubated with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039).
Expression of Leptin Receptor in mouse olfactory bulb.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Leptin Receptor immunoreactivity (green) appears in the mitral layer (M). B. Pre-incubation of the antibody with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039), suppressed staining. Cell nuclei are stained with DAPI (blue). M = mitral layer, G = glomerulus.
Expression of Leptin Receptor in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Leptin Receptor immunoreactivity (green) appears in the pyramidal layer (P). B. Pre-incubation of the antibody with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Leptin Receptor in rat dorsal root ganglion (DRG).Immunohistochemical staining of perfusion-fixed frozen rat DRG sections with Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Leptin Receptor immunoreactivity (green) appears in cell outlines (arrows). B. Pre-incubation of the antibody with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EDDGMKWLRIPSNVK, corresponding to amino acid residues 777 - 791 of mouse LEPR (Accession P48356).
Accession (Uniprot) Number P48356
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
