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Ligustilide

(E)-Ligustilide
An Activator of TRPA1 Channels
Cat #: L-170
Alternative Name (E)-Ligustilide
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Angelica sinensis.
    Source Natural
    MW: 190.2
    Purity: >95%
    Form Lyophilized powder.
    Effective concentration 10-200 µM.
    Structure
    Chemical name (3Z)-3-butylidene-4,5-dihydro-2-benzofuran-1-one.
    Molecular formula C12H14O2.
    CAS No.: 4431-01-0.
    PubChem CID 5319022
    Activity Ligustilide activates TRPA1 with an EC50 of 44 µM1.
    References-Activity
    1. Zhong, J. et al. (2011) Pfulgers Arch. 462, 841.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Up 100 mM in DMSO and 50 mM in Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Ligustilide activates TRPA1 channels expressed in HEK-293 cells. 
      Alomone Labs Ligustilide activates TRPA1 channels expressed in HEK-293 cells. 
      Dose-response curve for the activation of human TRPA1 expressing HEK-293 cells loaded with Calcium 5 dye for changes in intracellular Ca2+ measurements. Calcium signal was apparent with 200 µM Ligustilide (#L-170).
    References - Scientific background
    1. Cvetkov, T.L. et al. (2011) J. Biol. Chem. 286, 38168.
    2. Kuang, X. et al. (2008) Pharmacol. Biochem. Behav. 88, 213.
    3. Zhong, J. et al. (2011) Pfulgers Arch. 462, 841.
    4. Zhu, X. et al. (2015) J. Ethnopharmacol. 3, 86.
    Scientific background

    Transient receptor potential ankyrin 1 (TRPA1) is a non-selective ion channel, which is expressed in nociceptor sensory neurons and transduces chemical, inflammatory, and neuropathic pain signals. Numerous non-reactive compounds and electrophilic compounds, such as endogenous inflammatory mediators and exogenous pungent chemicals, can activate TRPA11.

    Ligustilide is a natural agonist of TRPA1 channels produced from the Umbelliferae plant Angelica sinensis with an effective concentration of 10-200 μM and an EC50 of 44 μM when binding to TRPA1. Ligustilide has a significant neuroprotective effect in transient forebrain ischemia and permanent cerebral focal ischemia. In a rat assay demonstrating ischemic damage, Ligustilide shortened mean escape time compared to the control group and prevented the effects caused by ligation of both common carotids. Ligustilide also increases choline acetyltransferase activity in ischemic brain tissues and might have therapeutic potential in the treatment of vascular-cerebral pathologies2. Ligustilide also plays a role in the prevention of cell autophagy. In C2C12 cells, ligustilide causes a decrease in the production TNF-α induced autophagosomes3. Interestingly, Ligustilide can be found in various drugs used in traditional Chinese medicine such as Danzhi Xiaoyao San. This substance ameliorates depressive behavior via upregulation of serotonin production thus making Ligustilide a research candidate for psychological disorders4.

    Target TRPA1 channels
    Last update: 23/08/2020

    Ligustilide (#L-170) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use
    Shipping and Ordering information