LPAR4/P2Y9 (extracellular) Blocking Peptide (#BLP-LR034) is the original antigen used for immunization during Anti-LPAR4 (P2Y9) (extracellular) Antibody (#ALR-034) generation. The blocking peptide binds and ‘blocks’ Anti-LPAR4/P2Y9 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes:
- Expression of LPA4 receptor in rat and mouse brainImmunohistochemical staining of rat choroid plexus and mouse hippocampal CA3 region using Anti-LPAR4 (P2Y9) (extracellular) Antibody (#ALR-034), (1:400). A. LPA4 receptor staining (red) in rat choroid plexus appears in astrocytes (vertical arrows) and in cells of the choroid plexus (horizontal arrow). C. LPA4 receptor staining (red) in mouse hippocampal CA3 region is detected in astrocytes (arrows). B, D. Nuclear staining using DAPI as the counterstain (blue).
- (C)DRRFIDFQFQDLNS, corresponding to amino acid residues 3-16 of mouse LPA4 receptor (Accession Q8BLG2).