LRRTM1 (extracellular) Blocking Peptide (#BLP-NR141) is the original antigen used for immunization during Anti-LRRTM1 (extracellular) Antibody (#ANR-141) generation. The blocking peptide binds and ‘blocks’ Anti-LRRTM1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain (lanes 1 and 3) and rat hippocampus (lanes 2 and 4) lysates:
- Expression of LRRTM1 in rat cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LRRTM1 (extracellular) Antibody (#ANR-141), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. LRRTM1 staining (green) in rat temporal cortex is detected in neuronal profiles (arrows). Cell nuclei are stained with DAPI (blue).
- (C)GRYDANLQ(S)ASPE, corresponding to amino acid residues 334-346 of mouse LRRTM1 (Accession Q8K377).