Anti-LRRTM1 (extracellular) Antibody

Leucine-rich repeat transmembrane neuronal protein 1
Cat #: ANR-141
Alternative Name Leucine-rich repeat transmembrane neuronal protein 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
      • Peptide (C)GRYDANLQ(S)ASPE, corresponding to amino acid residues 334-346 of mouse LRRTM1 (Accession Q8K377). Extracellular, N-terminus.
    Accession (Uniprot) Number Q8K377
    Gene ID 74342
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse and rat – 12/13 amino acid residues identical; human – 11/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
      • Western blot analysis of mouse brain (lanes 1 and 3) and rat hippocampus (lanes 2 and 4) lysates:
        1,2. Anti-LRRTM1 (extracellular) Antibody (#ANR-141), (1:400).
        3,4. Anti-LRRTM1 (extracellular) Antibody, preincubated with the control peptide antigen.
      • Expression of LRRTM1 in rat cortex
        Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LRRTM1 (extracellular) Antibody (#ANR-141), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. LRRTM1 staining (green) in rat temporal cortex is detected in neuronal profiles (arrows). Cell nuclei are stained with DAPI (blue).
      • Leucine rich repeat transmembrane neuronal 1 (LRRTM1) is a synaptogenic, cell adhesion protein that contributes to the regulation of synapse density and synaptic transmission. The LRRTM family in mammals includes four postsynaptic proteins, which have been shown to interact with presynaptic neurexins and heparan sulphate proteoglycan glypican on the presynaptic side1

        In human and mouse genomes, LRRTM1 is encoded by a single exon and is predominantly expressed in the nervous system. LRRTM1 is a single-membrane-spanning transmembrane protein and its structure contains ten N-terminal LRR repeats, a single pass transmembrane domain, and a cytoplasmic C-terminal tail comprising a PDZ-binding motif1.

        Mutations in human genes encoding the LRRTM proteins are implicated in cognitive disorders. In particular, LRRTM1 is associated to human handedness and schizophrenia1,2. Studies on Lrrtm1 knockout mice shows deficits in behavioral responses to stressful situations and novel objects, spatial memory and social discrimination deficits3. LRRTM1 mutant mice display impairment in visual behaviors, suggesting a functional role of LRRTM1 in retinothalamic convergence in vision4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/07/2019

    Anti-LRRTM1 (extracellular) Antibody (#ANR-141) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRTM1 from human, rat, and mouse samples.

    For research purposes only, not for human use