Overview
Type: Synthetic peptide
Form: Lyophilized powder
MC1 Receptor Blocking Peptide (#BLP-MR020) is the original antigen used for immunization during Anti-MC1 Receptor Antibody (#AMR-020) generation. The blocking peptide binds and ‘blocks’ Anti-MC1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6):1,2,3. Anti-MC1 Receptor Antibody (#AMR-020), (1:500).
4,5,6. Anti-MC1 Receptor Antibody, preincubated with MC1 Receptor Blocking Peptide (#BLP-MR020).
Western blot analysis of rat adrenal lysate:1. Anti-MC1 Receptor Antibody (#AMR-020), (1:400).
2. Anti-MC1 Receptor Antibody, preincubated with MC1 Receptor Blocking Peptide (#BLP-MR020).
Expression of MC1R in normal skin and melanomaImmunohistochemical staining of paraffin embedded normal skin and melanoma sections using Anti-MC1 Receptor Antibody (#AMR-020) (1:100). MCR1 staining (red-brown color is highly specific in A. epidermal cells, B. eccrine sweat gland cells and C. melanoma cells. Color reaction was obtained with DAB. Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R (Accession Q01726).
Accession (Uniprot) Number Q01726
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
