MC1 Receptor Blocking Peptide (#BLP-MR020) is the original antigen used for immunization during Anti-MC1 Receptor Antibody (#AMR-020) generation. The blocking peptide binds and ‘blocks’ Anti-MC1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6):
- Western blot analysis of rat adrenal lysate:
- Expression of MC1R in normal skin and melanomaImmunohistochemical staining of paraffin embedded normal skin and melanoma sections using Anti-MC1 Receptor Antibody (#AMR-020) (1:100). MCR1 staining (red-brown color is highly specific in A. epidermal cells, B. eccrine sweat gland cells and C. melanoma cells. Color reaction was obtained with DAB. Hematoxilin is used as the counterstain.
- (C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R (Accession Q01726).