MCT2 Blocking Peptide (#BLP-MT012) is the original antigen used for immunization during Anti-MCT2 Antibody (#AMT-012) generation. The blocking peptide binds and ‘blocks’ Anti-MCT2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse testis membranes:
- Western blot analysis of rat brain lysate (lanes 1 and 3) and rat testis membranes (lanes 2 and 4):
- Expression of MCT2 in mouse brain sectionsImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MCT2 Antibody (#AMT-012), (1:1000). A. MCT2 staining in mouse cortex sections (red) appears in neuronal outlines (arrows). B. MCT2 staining in dorsal pons brain stem regions (red) appears in neuronal outlines (horizontal arrows) and in fibers (vertical arrows). 4th V = fourth ventricle. Cell nuclei are stained with DAPI (blue).
- (C)KEMEALSRSKQDD, corresponding to amino acid residues 456-468 of rat MCT2 (Accession Q63344).