MCT4/SLC16A3 Blocking Peptide (#BLP-MT014) is the original antigen used for immunization during Anti-MCT4 (SLC16A3) Antibody (#AMT-014) generation. The blocking peptide binds and ‘blocks’ Anti-MCT4/SLC16A3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):
- (C)REVEHFLKAEPEK, corresponding to amino acid residues 447-459 of rat MCT4 (Accession O35910).