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Certificate of Analysis

Mechanosensitive TRP Channel Antibody Explorer Kit

A Screening Package of Mechanosensitive TRP Channel Antibodies Economically Priced

Cat #: AK-305
Size: 18 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-TRPA1 (extracellular) Antibody Cat #: ACC-037

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1 (Accession O75762). 1st extracellular loop.
Homology
Bovine - identical; canis - 13/14 amino acid residues identical; rat - 12/14 amino acid residues identical; mouse - 9/14 amino acid residues identical.

TRPA1 (extracellular) Blocking Peptide Cat #: BLP-CC037

Size: 40 µg

Anti-TRPC1 Antibody Cat #: ACC-010

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human TRPC1 (Accession P48995). Intracellular.
Homology
Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Xenopus Laevis - 11/14 amino acid residues identical.

TRPC1 Blocking Peptide Cat #: BLP-CC010

Size: 40 µg

Anti-TRPC1 (extracellular) Antibody Cat #: ACC-118

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)SMGQMLQDFGK, corresponding to amino acid residues 495-505 of rat TRPC1 (Accession Q9QX01). 2nd extracellular loop.
Homology
Human, mouse - identical.

TRPC1 (extracellular) Blocking Peptide Cat #: BLP-CC118

Size: 40 µg

Anti-TRPC6 Antibody Cat #: ACC-017

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143). Intracellular, N-terminus.
Homology
Rat - identical; human - 15/16 amino acid residues identical.

TRPC6 Blocking Peptide Cat #: BLP-CC017

Size: 40 µg

Guinea pig Anti-TRPC6 Antibody Cat #: ACC-017-GP (formerly AGP-002)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143). Intracellular, N-terminus.
Homology
Rat - identical; human - 15/16 amino acid residues identical.

TRPC6 Blocking Peptide Cat #: BLP-CC017

Size: 40 µg

Anti-TRPC6 (extracellular) Antibody Cat #: ACC-120

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6 (Accession Q61143). 2nd extracellular loop.
Homology
Rat, human - identical

TRPC6 (extracellular) Blocking Peptide Cat #: BLP-CC120

Size: 40 µg

Anti-TRPP1 (PKD2) Antibody Cat #: ACC-052

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 of human TRPP1 (Accession Q13563). Intracellular, C-terminus.
Homology
Mouse - 12/14 amino acid residues identical; rat - 11/14 amino acid residues identical.

TRPP1/PKD2 Blocking Peptide Cat #: BLP-CC052

Size: 40 µg

Anti-TRPV4 Antibody Cat #: ACC-034

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.
Homology
Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.

TRPV4 Blocking Peptide Cat #: BLP-CC034

Size: 40 µg

Anti-TRPV4 (extracellular) Antibody Cat #: ACC-124

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, R
Immunogen
Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
Homology
Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.

TRPV4 (extracellular) Blocking Peptide Cat #: BLP-CC124

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
A Guinea pig polyclonal antibody (#ACC-017-GP) is included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
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