Overview
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-mGluR1 (extracellular) Antibody (#AGC-006) and Anti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody (#AGC-006-AG). Intended to be used as a negative control.
Applications: bl
For research purposes only, not for human use
Applications
Applications
Blocking
Western blot analysis of rat brain lysate:1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200).
2. Anti-mGluR1 (extracellular) Antibody, preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).
Western blot analysis of mouse brain lysate:1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200).
2. Anti-mGluR1 (extracellular) Antibody, preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).
Multiplex staining of mGluR1 and TRPC3 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse cerebellum sections using Anti-TRPC3-ATTO Fluor-594 Antibody (ACC-016-AR), (1:60) and Anti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody (#AGC-006-AG), (1:60). A. TRPC3 staining (red). B. mGluR1 staining (green). C. Merge of the two images suggests extensive co-localization in Purkinje cells (vertical arrows). Note expression of mGluR1 in Purkinje dendrites (horizontal arrow) but not of TRPC3. Cell nuclei are stained with DAPI (blue).
Expression of mGluR1 in rat cerebellumImmunohistochemical staining of rat cerebellum frozen sections with Anti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody (#AGC-006-AG) (1:20). Staining (in green) appears in cerebellar Purkinje cells (arrows) and in the molecular layer.
Properties
Properties
Sequence
- (C)HEGVLNIDDYKIQMNK, corresponding to amino acids 501-516 of rat mGluR1 (Accession P23385).
Accession (Uniprot) Number P23385
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Shipping and Ordering information
