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Anti-mGluR1 (extracellular) Antibody

Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13
Cat #: AGC-006
Alternative Name Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)HEGVLNIDDYKIQMNK, corresponding to amino acids 501-516 of rat mGluR1 (Accession P23385). Extracellular, N-terminus.
Accession (Uniprot) Number P23385
Gene ID 24414
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human, dog - identical.
RRID AB_2039984.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ip, lci, wb
May also work in: ih*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200).
    2. Anti-mGluR1 (extracellular) Antibody, preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).
  • Western blot analysis of mouse brain lysate:
    Western blot analysis of mouse brain lysate:
    1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200).
    2. Anti-mGluR1 (extracellular) Antibody, preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).
Immunoprecipitation
  • Immunoprecipitation of rat brain lysate:
    Immunoprecipitation of rat brain lysate:
    1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) Antibody (#AGC-006).
    2. Cell lysate + protein A beads + pre-immune rabbit serum.
    3. Cell lysate.

    Black arrow indicates the mGluR1 protein (glycosylated form) while the red arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-mGluR1 (extracellular) Antibody.
Indirect flow cytometry
  • Cell surface detection of mGluR1 in live intact Jurkat cells:
    Cell surface detection of mGluR1 in live intact Jurkat cells:
    ___ Unstained cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-mGluR1 (extracellular) Antibody (#AGC-006), (10 µg) + goat-anti-rabbit-FITC.
Live cell imaging / Immunocytochemistry
  • Expression of mGluR1 in rat C6 glioma cells
    Expression of mGluR1 in rat C6 glioma cells
    Cell surface detection of mGluR1 in live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were stained with Hoechst 33342 (blue).
References
  1. Kew, J.N. and Kemp, J.A. (2005) Psychopharmacology (Berl.) 179, 4.
  2. Ferraguti, F. and Shigemoto, R. (2006) Cell Tissue Res. 326, 483.
  3. Dhami, G.K. and Ferguson, S.S. (2006) Pharmacol. Ther. 111, 260.
Scientific background

L-Glutamate is the major excitatory neurotransmitter in the central nervous system. It operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.

Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus and an intracellular C-terminus. The N-terminus domain of Group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.

mGluR1 and mGluR5 receptors signal through Gq/G11, that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. Additional downstream signaling pathways include the activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins have also been reported.

mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.

The mGluR1 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson’s and Huntington’s disease.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-mGluR1 (extracellular) Antibody (#AGC-006) is a highly specific antibody directed against the extracellular N-terminus domain of rat mGluR1. The antibody can be used in western blot and immunocytochemistry applications, and recognizes mGluR1 in rat, mouse and human samples.

For research purposes only, not for human use
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