mGluR3 (extracellular) Blocking Peptide (#BLP-GC012) is the original antigen used for immunization during Anti-mGluR3 (extracellular) Antibody (#AGC-012) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Expression of mGluR3 in rat brain stemImmunohistochemical staining of frozen brain stem sections using Anti-mGluR3 (extracellular) Antibody (#AGC-012), (1:100) followed by goat anti-rabbit AlexaFluor-555 secondary antibody (red). Staining is present in neuronal cell bodies (arrows) in the brainstem nuclei. Hoechst 33342 is used as the counterstain (blue).
- (C)GDHNFMRREIKIEGD, corresponding to amino acids 24-38 of rat mGluR3 (Accession P31422).