Overview
Type: Synthetic peptide
Form: Lyophilized powder
MRS2 Blocking Peptide (#BLP-NT148) is the original antigen used for immunization during Anti-MRS2 Antibody (#ANT-148) generation. The blocking peptide binds and ‘blocks’ Anti-MRS2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat testis (lanes 1 and 4) and kidney lysates (lanes 2 and 5) and heart membrane (lanes 3 and 6):1-3. Anti-MRS2 Antibody (#ANT-148), (1:400).
4-6. Anti-MRS2 Antibody, preincubated with MRS2 Blocking Peptide (#BLP-NT148).
Expression of MRS2 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-MRS2 Antibody (#ANT-148), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA3 region, showed MRS2 immunoreactivity (green) in the Pyramidal layer (P, arrows). B. Pre-incubation of the antibody with MRS2 Blocking Peptide (#BLP-NT148), suppressed staining. Cell nuclei are stained with DAPI (blue). P = Pyramidal layer.
Properties
Sequence
- (C)DPKHSSVDRSKLH, corresponding to amino acid residues 211-223 of rat MRS2 magnesium transporter (Accession Q9ET09).
Accession (Uniprot) Number Q9ET09
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
