Cat #: BLP-NX005
Type: Synthetic peptide
Form: Lyophilized powder
Na+/H+ Exchanger 5/NHE-5 Blocking Peptide (#BLP-NX005) is the original antigen used for immunization during Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody (#ANX-005) generation. The blocking peptide binds and ‘blocks’ Anti-Na+/H+ Exchanger 5/NHE-5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody (#ANX-005), (1:200).
3,4. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody, preincubated with Na+/H+ Exchanger 5/NHE-5 Blocking Peptide (#BLP-NX005).
- CAEKELPWKSGQGD, corresponding to amino acid residues 759-772 of rat NHE-5 (Accession Q9Z0X2).
Accession (Uniprot) Number Q9Z0X2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.