Neogenin (extracellular) Blocking Peptide (#BLP-NR195) is the original antigen used for immunization during Anti-Neogenin (extracellular) Antibody (#ANR-195) generation. The blocking peptide binds and ‘blocks’ Anti-Neogenin (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat spleen membranes (lanes 2 and 4):
- Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 2 and 4):
- Expression of Neogenin in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neogenin (extracellular) Antibody (#ANR-195), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Neogenin immunoreactivity (green) appears in cortical neurons in layer 3 (arrows). B. Pre-incubation of the antibody with Neogenin (extracellular) Blocking Peptide (BLP-NR195), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Peptide (C)KIRYRKASRKSDVTE, corresponding to amino acid residues 701 - 715 of mouse Neogenin (Accession P97798).