Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003) is the original antigen used for immunization during Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotrophin 3/NT-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (100 ng):
- Expression of Neurotrophin 3 in mouse spinal cordImmunohistochemical staining of perfusion-fixed frozen mouse spinal cord sections with Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 (green). The same sections were stained with mouse anti-neurofilament 200 (NF) (red). Merge of the two images demonstrates the presence of NT-3 in gray matter which colocalizes with NF only in the soma of some neurons (arrow).
- Rat hippocampus (1:300) (Ramos Languren, L.E. and Escobar, M.L. (2013) Eur. J. Neurosci. 37, 1248).
- GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor) (Accession P20783).