Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003) is the original antigen used for immunization during Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotrophin 3/NT-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (100 ng):1. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:200)2. Anti-Neurotrophin 3 (NT-3) Antibody, preincubated with Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003).
Expression of Neurotrophin 3 in mouse spinal cordImmunohistochemical staining of perfusion-fixed frozen mouse spinal cord sections with Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 (green). The same sections were stained with mouse anti-neurofilament 200 (NF) (red). Merge of the two images demonstrates the presence of NT-3 in gray matter which colocalizes with NF only in the soma of some neurons (arrow).- Rat hippocampus (1:300) (Ramos Languren, L.E. and Escobar, M.L. (2013) Eur. J. Neurosci. 37, 1248).
Properties
Sequence
- GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor) (Accession P20783).
Accession (Uniprot) Number P20783
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 0.5 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
