NKCC1/SLC12A2 (extracellular) Blocking Peptide (#BLP-NT071) is the original antigen used for immunization during Anti-NKCC1 (SLC12A2) (extracellular) Antibody (#ANT-071) generation. The blocking peptide binds and ‘blocks’ Anti-NKCC1/SLC12A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat lung lysate (lanes 3 and 6):
- Expression of NKCC1 in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NKCC1 (SLC12A2) (extracellular) Antibody (#ANT-071), (1:100). A. NKCC1 staining (red) is detected in the cerebellar molecular layer (Mol). B. DAPI counterstain (blue) reveals the outline of cerebellar layers. C. Merge of the two images.
- (C)RRQAMKEMSIDQAK, corresponding to amino acid residues 828-841 of human NKCC1 (Accession P55011).