Overview
Type: Synthetic peptide
Form: Lyophilized powder
NPY1R Blocking Peptide (#BLP-NR021) is the original antigen used for immunization during Anti-NPY1R Antibody (#ANR-021) generation. The blocking peptide binds and ‘blocks’ Anti-NPY1R primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human Jurkat acute T cell leukemia (lanes 1 and 4), human K562 chronic myelogeneous leukemia (lanes 2 and 5), and rat RBL basophilic leukemia (lanes 3 and 6) cell lysates and rat brain lysates (lanes 7 and 8):1,2,3,7. Anti-NPY1R Antibody (#ANR-021), (1:200).
4,5,6,8. Anti-NPY1R Antibody, preincubated with NPY1R Blocking Peptide (#BLP-NR021).
Expression of neuropeptide Y1 receptor in rat striatumImmunohistochemical staining of rat striatum using Anti-NPY1R Antibody (#ANR-021). A. NPY1R (red) appears in the striatal matrix (white asterisk) and in medium-size cells in the matrix (arrows). B. Parvalbumin (green) appears in the striatal matrix. C. Confocal merge of NPY1R and parvalbumin indicates that NPY1R is restricted to granule cells. DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)RLKRRNNMMDKMRDNK, corresponding to amino acid residues 237-252 of human NPY1R (Accession P25929).
Accession (Uniprot) Number P25929
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
