Overview
Cat #: BLP-NR115
Type: Synthetic peptide
Form: Lyophilized powder
NRG3 (extracellular) Blocking Peptide (#BLP-NR115) is the original antigen used for immunization during Anti-NRG3 (extracellular) Antibody (#ANR-115) generation. The blocking peptide binds and ‘blocks’ Anti-NRG3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-NRG3 (extracellular) Antibody (#ANR-115), (1:200).
3,4. Anti-NRG3 (extracellular) Antibody, preincubated with NRG3 (extracellular) Blocking Peptide (#BLP-NR115). - Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell lysate (lanes 2 and 4):1,2. Anti-NRG3 (extracellular) Antibody (#ANR-115), (1:200).
3,4. Anti-NRG3 (extracellular) Antibody, preincubated with NRG3 (extracellular) Blocking Peptide (#BLP-NR115).
Properties
Sequence
- (C)RFPGHRVPIRASPR, corresponding to amino acid residues 167-180 of mouse Neuregulin-3 (Accession O35181).
Accession (Uniprot) Number O35181
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.