Oxytocin Receptor Blocking Peptide (#BLP-VR013) is the original antigen used for immunization during Anti-Oxytocin Receptor Antibody (#AVR-013) generation. The blocking peptide binds and ‘blocks’ Anti-Oxytocin Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane (1:200), rat testis and mouse testis lysates (1:400):
- Expression of Oxytocyn Receptor in rat midbrain.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Oxytocin Receptor Antibody (#AVR-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Oxytocyn Receptor immunoreactivity (green) appears in neuronal profiles (arrows) in the substantia nigra pars compacta (SNC). B. Pre-incubation of the antibody with Oxytocin Receptor Blocking Peptide (#BLP-VR013), suppressed staining. Cell nuclei are stained with DAPI (blue).
- CSARYLKGSRPGE, corresponding to amino acid residues 346-358 of rat Oxtr (Accession P70536).