P2X1 Receptor (extracellular) Blocking Peptide (#BLP-PR022) is the original antigen used for immunization during Anti-P2X1 Receptor (extracellular) Antibody (#APR-022) generation. The blocking peptide binds and ‘blocks’ Anti-P2X1 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
- Expression of P2X1 Receptor in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-P2X1 Receptor (extracellular) Antibody (#APR-022). A. Most of P2RX1 labeling (green) appears in fine processes in the molecular layer (MOL) and in Purkinje cells (arrows show examples). B. DAPI is used as the counterstain (blue). C. Merge of A and B.
- CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575).