Anti-P2X1 Receptor (extracellular) Antibody

P2RX1, P2X purinoceptor 1
    Cat #: APR-022
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575). Extracellular loop.
    Accession (Uniprot) Number P51575
    Gene ID 5023
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, lci, wb
    May also work in: ip
    Western blot
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    1,2. Anti-P2X1 Receptor (extracellular) Antibody (#APR-022), (1:200).
    3,4. Anti-P2X1 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of P2X1 Receptor in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-P2X1 Receptor (extracellular) Antibody (#APR-022). A. Most of P2RX1 labeling (green) appears in fine processes in the molecular layer (MOL) and in Purkinje cells (arrows show examples). B. DAPI is used as the counterstain (blue). C. Merge of A and B.
    Indirect flow cytometry
    Indirect flow cytometry analysis of MEG-O1 cells:
    ___ Unstained cells.
    ___ Cells + Anti-P2X1 Receptor (extracellular) Antibody (#APR-022), (10 µg antibody/1x106 cells).
    The control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    Expression of P2X1 Receptor in human SH-SYS5 cells
    Immunocytochemical staining of intact living human SH-SYS5 cells. A. Extracellular staining of cells with Anti-P2X1 Receptor (extracellular) Antibody (#APR-022), (1:50) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining DAPI as the counterstain. C. Merged images of A and B.
    References
    1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
    2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
    3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
    4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
    5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
    6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152.
    7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
    8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
    9. Kim, M. et al. (2001) EMBO J. 20, 6347.
    10. Burnstock, G. (2001) Trends Pharmacol. Sci. 22, 182.
    11. Oury, C. et al. (2003) Blood 101, 3969.
    Scientific background

    The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

    The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

    Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned1-3. All P2X receptor subtypes share the same structure of intracellular N- and C-termini two membrane-spanning domains and a large extracellular loop.

    All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex4-9.

    The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous systems, while the P2X7 receptor is predominantly found in cells of the immune system4. The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis3,4,10,11.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 29/11/2018

    Anti-P2X1 Receptor (extracellular) Antibody (#APR-022) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize P2X1 receptor from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
    Indirect flow cytometry citations
    1. Human eosinophils.
      Wright, A. et al. (2016) J. Immunol. 196, 4877.
    Related Products
      • Products for control experiments
        1. Anti-P2X1 Receptor Antibody (#APR-001). This antibody recognizes a different epitope and thus can also be used as a control for detecting P2X1.