P2X2 Receptor Blocking Peptide (#BLP-PR003) is the original antigen used for immunization during Anti-P2X2 Receptor Antibody (#APR-003) generation. The blocking peptide binds and ‘blocks’ Anti-P2X2 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of differentiated (lanes 1 and 3) and non-differentiated (lanes 2 and 4) PC12 cell lysates:
- Expression of P2X2 receptor in rat neocortexImmunohistochemical staining of rat neocortex with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in axonal processes (arrows) that ascend toward the upper cortical layers (asterisk). DAPI is used as the counterstain (blue).
- Expression of P2X2 receptor in rat hippocampusImmunohistochemical staining of rat hippocampus with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in the apical dendrites (arrows) that extend into the striatum radiatum (asterisk). DAPI is used as the counterstain (blue).
- Mouse ilieum (1:200) (Ren, J. et al. (2003) J. Physiol. 552, 809.).
- (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor (Accession P49653).