Anti-P2X2 Receptor Antibody

P2X purinoceptor 2, P2RX2
    Cat #: APR-003
    Alternative Name P2X purinoceptor 2, P2RX2
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor (Accession P49653). Intracellular, C-terminus.
    • Anti-P2X2 Receptor Antibody
    Accession (Uniprot) Number P49653
    Gene ID 114115
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Guinea pig - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.
    RRID AB_2040054.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.9 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, wb
    Western blot
    • Anti-P2X2 Receptor Antibody
      Western blot analysis of differentiated (lanes 1 and 3) and non-differentiated (lanes 2 and 4) PC12 cell lysates:
      1,2. Anti-P2X2 Receptor Antibody (#APR-003), (1:200).
      3,4. Anti-P2X2 Receptor Antibody, preincubated with the control peptide antigen.
    Immunoprecipitation
    • Rat brain membranes (2 μg) (Masin, M. et al. (2006) J. Biol. Chem. 281, 4100.).
    Immunohistochemistry
    • Anti-P2X2 Receptor Antibody
      Expression of P2X2 receptor in rat neocortex
      Immunohistochemical staining of rat neocortex with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in axonal processes (arrows) that ascend toward the upper cortical layers (asterisk). DAPI is used as the counterstain (blue).
    • Anti-P2X2 Receptor Antibody
      Expression of P2X2 receptor in rat hippocampus
      Immunohistochemical staining of rat hippocampus with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in the apical dendrites (arrows) that extend into the striatum radiatum (asterisk). DAPI is used as the counterstain (blue).
    • Mouse ilieum (1:200) (Ren, J. et al. (2003) J. Physiol. 552, 809.).
    Immunocytochemistry
    • Cultured rat myeteric cells (1:200) (Ohta, T. et al. (2005) Am. J. Physiol. 289, G935.).
    Indirect flow cytometry
    • Human CD4+ and ckit+ cells (Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.).
    • The control antigen is not suitable for this application.
    References
    1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
    2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
    3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
    4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
    5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
    6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152.
    7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
    8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
    9. Kim, M. et al. (2001) EMBO J. 20, 6347.
    10. Chizh, B.A. and Illes.P. (2001) Pharmacol. Rev. 53, 553.
    Scientific background

    The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

    The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

    Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9

    The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.

    mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-P2X2 Receptor AntibodyExpression of P2X2 receptor in rat SCN.Immunocytochemical staining of rat suprachiasmatic nuclei (SCN) cell culture. Fixed and permeabilized cells were stained using Anti-P2X2 Receptor Antibody (#APR-003). P2RX2 immunoreactivity (red) is observed in punctate structures in the SCN cell culture. Arrowheads indicate example structures that are double-labeled with P2RX2 and synapsin I (green). Dapi is used to label cell nuclei. Scale bar, 20 μm.Adapted from Bhattacharya, A. et al. (2013) with permission of The Society for Neuroscience.
    Last update: 27/02/2020

    Anti-P2X2 Receptor Antibody (#APR-003) is a highly specific antibody directed against an intracellular epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X2 receptor from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Immunohistochemical staining of mouse inner ear sections. Tested in P2X2R-/- mice.
      Takimoto, Y. et al. (2018) Neuroscience 386, 41.
    Western blot citations
    1. Mouse kidney lysates (1:500).
      de Baaij, J.H.F. et al. (2016) PLoS ONE 11, e0156803.
    2. HEK293-TSA 201 transfected cell lysates (1:5000).
      Allsopp, R. and Evans, R.J. (2015) J. Biol. Chem. 290, 14556.
    Immunoprecipitation citations
    1. Rat brain membranes (2 μg).
      Masin, M. et al. (2006) J. Biol. Chem. 281, 4100.
    Immunohistochemistry citations
    1. Mouse vestibular labyrinth sections.
      Jeong, J. et al. (2020) Hearing Res. 386, 107860.
    2. Mouse inner ear sections. Also tested in P2X2R-/- mice.
      Takimoto, Y. et al. (2018) Neuroscience 386, 41.
    3. Mouse olfactory bulb sections.
      Stratford, J.M. et al. (2017) Comp. Neurol. 525, 271.
    4. Mouse medulla sections.
      Breza, J.M. and Travers, S.P. (2016) Chem. Senses 41, 515.
    5. Mouse tongue sections (1:300).
      Voigt, A. et al. (2015) J. Neurosci. 35, 9717.
    6. Rat spinal cord sections.
      Vazquez-Villoldo, N. et al. (2014) Glia 62, 171.
    7. Mouse brainstem sections (1:500).
      Ganchrow, D. et al. (2014) J. Comp. Neurol. 522, 1565.
    8. Rat paraganglia (1:500).
      Piskuric, N.A. et al. (2013) Exp. Physiol. 99, 248.
    9. Mouse ilieum (1:200).
      Ren, J. et al. (2003) J. Physiol. 552, 809.
    Immunocytochemistry citations
    1. Human ESC NPs (1:100).
      Forostyak, O. et al. (2013) Stem Cells Dev. 22, 1506.
    2. Rat suprachiasmatic nuclei (SCN) culture.
      Bhattacharya, A. et al. (2013) J. Neurosci. 33, 8035.
    3. Cultured rat myeteric cells (1:200).
      Ohta, T. et al. (2005) Am. J. Physiol. 289, G935.
    Immunofluorescence citations
    1. Mouse vestibular labyrinth sections.
      Jeong, J. et al. (2020) Hearing Res. 386, 107860.
    Indirect flow cytometry citations
    1. Human CD4+ and ckit+ cells.
      Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.
    More product citations
    1. Housley, G.D. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 7494.
    2. Kim, M. et al. (2001) EMBO J. 20, 6347.
    3. Kim, M. et al. (2001) J. Biol. Chem. 276, 23262.
    4. Rubio, M.E., and Soto, F. (2001) J. Neurosci. 21, 641.
    5. Atkinson, L. et al. (2000) Neuroscience 99, 683.
    6. D'Ambrosi, N. et al. (2000) J. Neuropharm. 39, 1083.
    7. Gitterman, D.P. and Evans, R.J. (2000) Br. J. Pharmacol. 131, 1561.
    8. Lewis, C.J., and Evans, R.J. (2000) Br. J. Pharmacol. 131, 1659.
    9. Vial, C. and Evans, R.J. (2000) Br. J. Pharmacol. 131, 1489.
    10. Zhang, M. et al. (2000) J. Physiol. 525.1, 143.
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