P2X3 Receptor (extracellular) Blocking Peptide (#BLP-PR026) is the original antigen used for immunization during Anti-P2X3 Receptor (extracellular) Antibody (#APR-026) generation. The blocking peptide binds and ‘blocks’ Anti-P2X3 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat DRG:
- Expression of P2RX3 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), followed by anti-rabbit-AlexaFluor-594 secondary antibody. P2RX3 staining (red) appears in neuronal cell bodies. DAPI was used as the counter stain (blue). A. X20 magnification. B. X40 magnification.
- (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654).