P2X5 Receptor Blocking Peptide (#BLP-PR005) is the original antigen used for immunization during Anti-P2X5 Receptor Antibody (#APR-005) generation. The blocking peptide binds and ‘blocks’ Anti-P2X5 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of P2RX5 in rat spinal cordImmunohistochemical staining of rat spinal cord frozen section using Anti-P2X5 Receptor Antibody (#APR-005), (1:100). P2RX5 staining (red) is specific for neurons in the anterior horn of the spinal cord. Hoechst 33342 is used as the counterstain.
- (C)RENAIVNVKQSQILHPVKT, corresponding to amino acid residues 437-455 of rat P2X5 receptor (Accession P51578).