P2Y1 Receptor (extracellular) Blocking Peptide (#BLP-PR021) is the original antigen used for immunization during Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y1 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates:
- Expression of P2RY1 in mouse hippocampusImmunohistochemical staining of mouse hippocampal CA3 region using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021). A. P2RY1 staining (red) appears in the pyramidal layer (arrows). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.
- SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900).