P2Y13 Receptor Blocking Peptide (#BLP-PR017) is the original antigen used for immunization during Anti-P2Y13 Receptor Antibody (#APR-017) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y13 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of P2RY13 in rat testisImmunohistochemical staining of paraffin embedded rat epidydimus section using Anti-P2Y13 Receptor Antibody (#APR-017), (1:50). Strong and high specific staining of the epithelial cells is shown. Universal Immuno-alkaline-phosphatase kit followed by New Fuchsin Substrate was used for color reaction (red). Hematoxilin is used as the counterstain.
- DRFLKIIRPLRNIFLK(C), corresponding to amino acid residues 119-134 of human P2RY13 (Accession Q9BPV8).