Anti-P2Y13 Receptor Antibody

P2Y purinoceptor 13, P2RY13, GPCR1, GPR86, GPR94
    Cat #: APR-017
    Alternative Name P2Y purinoceptor 13, P2RY13, GPCR1, GPR86, GPR94
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide DRFLKIIRPLRNIFLK(C), corresponding to amino acid residues 119-134 of human P2RY13 (Accession Q9BPV8). 2nd intracellular loop.
    • Anti-P2Y13 Receptor Antibody
    Accession (Uniprot) Number Q9BPV8
    Gene ID 53829
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 11/16 amino acid residues identical; rat - 10/16 amino acid residues identical.
    RRID AB_2040076.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, wb
    May also work in: ip*
    Western blot
    • Anti-P2Y13 Receptor Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-P2Y13 Receptor Antibody (#APR-017), (1:200).
      2. Anti-P2Y13 Receptor Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-P2Y13 Receptor Antibody
      Expression of P2RY13 in rat testis
      Immunohistochemical staining of paraffin embedded rat epidydimus section using Anti-P2Y13 Receptor Antibody (#APR-017), (1:50). Strong and high specific staining of the epithelial cells is shown. Universal Immuno-alkaline-phosphatase kit followed by New Fuchsin Substrate was used for color reaction (red). Hematoxilin is used as the counterstain.
    Immunocytochemistry
    • Mouse hippocampal neuronal culture (del Puerto, A. et al. (2011) J. Cell Sci125, 176.).
    Indirect flow cytometry
    • Rat osteoclasts (10 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
    • The control antigen is not suitable for this application.
    References
    1. Communi, D. et al. (2001) J. Biol. Chem. 276, 41479.
    2. Zhang, F.L. et al. (2002) J. Pharmacol. Exp. Ther. 301, 705.
    3. http://www.iuphar-db.org/DATABASE/ReceptorFamiliesForward?type=GPCR
    4. Wang, L. et al. (2005) Circ. Res. 96, 189.
    5. Wirkner, K. et al. (2004) Br. J. Pharmacol. 141, 141.
    Scientific background

    The P2Y family belongs to the G-protein coupled receptors superfamily. They are activated by extracellular nucleotides and modulate variety of physiological functions.

    A new member of this family was recently identified, the P2Y13 receptor.1,2  The P2Y13 receptor has significant similarity to the P2Y12 receptor (about 45% sequence identity) and together they form a distinct group structurally differ from other members of this family. P2Y13 receptor, like the P2Y12  receptor, is a high affinity receptor for the ADP nucleotide, coupled to the Gai class of G-proteins.1,2 The P2Y13 receptor is highly expressed at brain and spleen tissues as well as in the immune system. Lower expression was demonstrated in the testis, lung liver and other peripheral organs.1-3 

    Recently it has been shown that ADP activates a negative feedback pathway for ATP release from human red blood cells via the P2Y13 receptor.4

    P2Y13 receptor was also implicated in inhibition of N-type Ca2+ channels in neurons, exerting pre and/or post-synaptic modulatory action.5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-P2Y13 Receptor Antibody (#APR-017) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, indirect flow cytometry, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY13 in human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Rat heart lysate (1:200).
      Giachini, F.R. et al. (2014) PLoS ONE 9, e91890.
    Immunohistochemistry citations
    1. Mouse muscle sections.
      Guarracino, J.F. et al. (2016) Neuroscience 326, 31.
    Immunocytochemistry citations
    1. Human fibroblasts (1:25).
      Pinheiro, A.R. et al. (2013) J. Biol. Chem. 288, 27571.
    2. Mouse hippocampal neuronal culture.
      del Puerto, A. et al. (2011) J. Cell Sci. 125, 176.
    Indirect flow cytometry citations
    1. Rat osteoclasts (10 μg/ml).
      Alvarenga, E.C. et al. (2010) Bone 46, 355.
    More product citations
    1. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
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