P2Y14 Receptor (extracellular) Blocking Peptide (#BLP-PR018) is the original antigen used for immunization during Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y14 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat heart membranes (lanes 1 and 4), brain (lanes 2 and 5) and lung lysates (lanes 3 and 6):
- Mouse kidney sections (Azroyan, A. et al. (2015) PLoS ONE 10, e0121419.).
- Expression of P2Y14 in rat pancreas.Immunohistochemical staining of paraffin-embedded sections of rat pancreas with Anti-P2Y14 (extracellular) Antibody (#APR-018), (1:50), followed by SuperPicture HRP polymer. A. Staining in the rat pancreas showed P2Y14 immunoreactivity (brown) in endocrine cells of the Isles of Langerhans (arrows). Counterstain is Hematoxilin.
- CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2RY14 (Accession Q15391).