Anti-P2Y14 Receptor (extracellular) Antibody

P2Y purinoceptor 14, P2RY14, GPR105, UDP-glucose receptor
    Cat #: APR-018
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2RY14 (Accession Q15391). 2nd extracellular loop.
    Accession (Uniprot) Number Q15391
    Gene ID 9934
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 15/17 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, ih, wb
    May also work in: ic, ip, lci
    Western blot
    Western blot analysis of rat heart membranes (lanes 1 and 4), brain (lanes 2 and 5) and lung lysates (lanes 3 and 6):
    1-3. Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018), (1:200).
    4-6. Anti-P2Y14 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Mouse kidney sections (Azroyan, A. et al. (2015) PLoS ONE 10, e0121419.).
    Indirect flow cytometry
    5-10 µg antibody / 1x10cells.

    Mouse thymocytes (Kook, S.H. et al. (2013) Cell Death Dis. 4, e703.).
    References
    1. Chambers, J.K. et al. (2000) J. Biol. Chem. 275, 10767.
    2. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
    3. Skelton, L. et al. (2003) J. Immunol. 171, 1941.
    Scientific background

    P2Y14 belongs to the P2Y receptor family that is part of the G-protein coupled receptor superfamily. To date, eight functional mammalian P2Y receptors have been identified: P2Y1P2Y2P2Y4P2Y6P2Y11P2Y12P2Y13 and P2Y14.

    The P2Y receptor family mediates the effects of extracellular nucleotides such as ATP, ADP, UTP, and in the case of P2Y14, UDP-glucose. UDP-glucose, as with the other nucleotides, can be released to the extracellular milieu as the result of cell damage or by regulated non-lytic mechanisms.

    P2Y14 is expressed in several tissues including the brain, lung and several cells of hematopoietic origin such as T lymphocytes and dendritic cells.

    The exact function of this receptor in the different tissues has not been clearly established but an involvement in chemotaxis, maturation of immature dendritic cells and neuroimmune regulation has been suggested.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-P2Y14 Receptor (extracellular) Antibody
    Expression of P2Y14 receptor in mouse kidney.Immunohistochemical staining of mouse kidney sections using Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018). P2RY14 staining (green) is detected in intercalated cells in the cortex and co-localizes with V-ATPase B1 subunit (red).Adapted from Azroyan, A. et al. (2015) PLoS ONE 10, e0121419. with permission of PLoS.
    Last update: 14/02/2019

    Anti-P2Y14 (extracellular) Antibody (#APR-018) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus highly suited for detecting the receptor in living cells. It has been designed to recognize P2Y14 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
    Published figures using this product
    Expression of P2Y14 receptor in mouse kidney.
    Immunohistochemical staining of mouse kidney sections using Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018). P2Y14 receptor staining (green) is detected in intercalated cells only of the cortex (A) and medulla (B). P2Y14 receptor co-localizes with V-ATPase B1 subunit (red staining) in intercalated cells but not in distal tubule cells. Pre-incubation of the control peptide antigen, abolishes P2Y14 staining in both cortex (C) and medulla (D).
    Adapted from Azroyan, A. et al. (2015) with kind permission of Dr. Breton, S. of Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts, USA.
    Western blot citations
    1. Rat brain microvascular endothelial cell lysate (1:200).
      Li, F. et al. (2016) J. Ethnopharmacol. 185, 77.
    Immunohistochemistry citations
    1. Mouse kidney sections.
      Azroyan, A. et al. (2015) PLoS ONE 10, e0121419.
    Indirect flow cytometry citations
    1. Mouse thymocytes.
      Kook, S.H. et al. (2013) Cell Death Dis. 4, e703.
    Related Products