Overview
Cat #: BLP-PR102
Type: Synthetic peptide
Form: Lyophilized powder
P2Y2 Receptor (extracellular) Blocking Peptide (#BLP-PR102) is the original antigen used for immunization during Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y2 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse heart lysate (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat brain lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8):1-4. Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200).
5-8. Anti-P2Y2 Receptor (extracellular) Antibody, preincubated with P2Y2 Receptor (extracellular) Blocking Peptide (#BLP-PR102). - Expression of P2Y2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. P2RY2 immunoreactivity (green) in the hippocampal dentate gyrus appears in blood vessels (horizontal arrows) and in neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
Accession (Uniprot) Number P41232
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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