P2Y4 Receptor Blocking Peptide (#BLP-PR006) is the original antigen used for immunization during Anti-P2Y4 Receptor Antibody (#APR-006) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y4 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of P2RY4 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-P2Y4 Receptor Antibody (#APR-006). A. P2RY4 (red) appears in astrocytic processes (arrows). B. In contrast, P2RY4 does not appear in Purkinje neuronal dendrites stained for calbindin D28k (green) in the same brain section. This demonstrates the restriction of P2RY4 to astrocytic fibers.
- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
- (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4 (Accession O35811).