P2Y6 Receptor Blocking Peptide (#BLP-PR011) is the original antigen used for immunization during Anti-P2Y6 Receptor Antibody (#APR-011) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y6 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat heart membranes:
- Mouse paraffin heart section.
- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
- Expression of P2Y6 in rat cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y6 Receptor Antibody (#APR-011), (1:600), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. P2Y6 immunoreactivity (red) appears in cortical neurons (horizontal arrows) and astrocytes (vertical arrows). B. Pre-incubation of the antibody with P2Y6 Receptor Blocking Peptide (BLP-PR011), suppressed staining. Cell nuclei are stained with DAPI (blue).
- CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession Q63371).