Anti-P2Y6 Receptor Antibody

P2Y purinoceptor 6, P2RY6
    Cat #: APR-011
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession Q63371). Intracellular, C-terminus.
    Accession (Uniprot) Number Q63371
    Gene ID 117264
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, ip, wb
    Western blot
    Western blot analysis of rat heart membranes:
    1. Anti-P2Y6 Receptor Antibody (#APR-011), (1:200).
    2. Anti-P2Y6 Receptor Antibody, preincubated with the control peptide antigen.
    Immunoprecipitation
    Mouse theca intertitial cells (3 μl) (Vazquez Cuevas, F.G. et al. (2010) Reprod. Biol. Endocrinol. 8, 88.).
    Immunohistochemistry
    Mouse paraffin heart section.
    Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
    Immunocytochemistry
    Human P2Y6 SHSY-5Y transfected cells (1:500) (Apolloni, S. et al. (2010) Neurochem. Int. 56, 670.).
    Indirect flow cytometry
    Rat osteoclasts (6 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
    The control antigen is not suitable for this application.
    References
    1. Communi, D. et al. (2000) Cell Signal. 12, 351.
    2. Filippov, A.K. et al. (1999) Br. J. Pharmacol. 126, 1009.
    3. Chang, K. et al. (1995) J. Biol. Chem. 270, 26152.
    4. Malmsjo, M. et al. (2003) B.M.C. Pharmacol. 3, 4.
    Scientific background

    The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily.1,3

    P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels.1,2

    P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries.4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/02/2019

    Anti-P2Y6 Receptor Antibody (#APR-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY6 from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
    Western blot citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    Immunoprecipitation citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Mouse theca intertitial cells (3 μl).
      Vazquez Cuevas, F.G. et al. (2010) Reprod. Biol. Endocrinol. 8, 88.
    Immunohistochemistry citations
    1. Mouse brain sections (1:100).
      Ribeiro Xavier, A.L. et al. (2015) J. Neurosci. 35, 11848.
    2. Human eye tissue.
      Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
    Immunocytochemistry citations
    1. Human P2Y6 SHSY-5Y transfected cells (1:500).
      Apolloni, S. et al. (2010) Neurochem. Int. 56, 670.
    Indirect flow cytometry citations
    1. Mouse bone marrow cells.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat osteoclasts (6 μg/ml).
      Alvarenga, E.C. et al. (2010) Bone 46, 355.
    More product citations
    1. Balasubramanian, R. et al. (2013) Biochem. Pharmacol. 85, 991.
    2. Traves, P.G. et al. (2013) J. Immunol. 190, 4226.
    3. Yu, W. et al. (2013) FASEB J. 27, 1895.
    4. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
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