Overview
Type: Synthetic peptide
Form: Lyophilized powder
PACSIN1 Blocking Peptide (#BLP-IP023) is the original antigen used for immunization during Anti-PACSIN1 Antibody (#AIP-023) generation. The blocking peptide binds and ‘blocks’ Anti-PACSIN1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3), mouse brain (lanes 2 and 4), (1:200) and human brain neuroblastoma SH-SY5Y cell line lysates (lanes 5 and 6), (1:600):1, 2, 5. Anti-PACSIN1 Antibody (#AIP-023).
3, 4, 6. Anti-PACSIN1 Antibody, preincubated with PACSIN1 Blocking Peptide (#BLP-IP023).
Expression of PACSIN1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PACSIN1 Antibody (#AIP-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed PACSIN1 immunoreactivity (green) in some neurons (arrows) in the pyramidal layer (P). B. Pre-incubation of the antibody with PACSIN1 Blocking Peptide (BLP-IP023), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EWNPDLPHTAAKKEK corresponding to amino acid residues 301-315 of rat PACSIN1 (Accession Q9Z0W5).
Accession (Uniprot) Number Q9Z0W5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
