Overview
Type: Synthetic peptide
Form: Lyophilized powder
Pan Nav Blocking Peptide (#BLP-SC003) is the original antigen used for immunization during Anti-Pan NaV Antibody (#ASC-003) generation. The blocking peptide binds and ‘blocks’ Anti-Pan Nav primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Pan NaV Antibody (#ASC-003), (1:200).
2. Anti-Pan NaV Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).
Expression of NaV channels in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-Pan NaV Antibody (#ASC-003). A. NaV channels (red) appear in Purkinje cell bodies (vertical arrows) and in cells of the molecular layer (Mol), (horizontal arrows). B. Staining of Parvalbumin (green) in the same brain section. C. Confocal merge of NaV channels and Parvalbumin demonstrates the location of NaV channels to the cell body of Purkinje neurons.
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Guinea pig Anti-Pan NaV Antibody (#ASC-003-GP), (1:800).
3,4. Guinea pig Anti-Pan NaV Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).
Properties
Sequence
- TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774).
Accession (Uniprot) Number P04774
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
