Pan VDAC Blocking Peptide (#BLP-VC001) is the original antigen used for immunization during Anti-VDAC Antibody (#AVC-001) generation. The blocking peptide binds and ‘blocks’ Anti-Pan VDAC primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney (lanes 1 and 4), rat brain (lanes 2 and 5), mouse brain (lanes 3 and 6) membranes and human CCF-SSTGI brain astrocytoma (lanes 7 and 8) cell line lysate:
- (C)DGKNVNAGGHK, corresponding to amino acid residues of 264-274 of rat VDAC1 (Accession Q9Z2L0).