PAR4/F2RL3 (extracellular) Blocking Peptide (#BLP-PR034) is the original antigen used for immunization during Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034) generation. The blocking peptide binds and ‘blocks’ Anti-PAR4/F2RL3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates:
- Western blot analysis of human prostate carcinoma PC3 (lanes 1 and 4), and Human LNCaP prostate carcinoma (lanes 2 and 5), and human T cell leukemia Jurkat (lanes 3 and 6) cell lines:
- Expression of PAR-4 in rat lungImmunohistochemical staining of rat lung paraffin-embedded sections using Anti-PAR4 (F2RL3) (extracellular) Antibody (APR-034), (1:100). Note that staining is present in the respiratory epithelium of the bronchiole (blue arrow) as well as in the smooth muscle cells of the muscularis mucosae (red arrow). Hematoxilin is used as the counterstain.
- Expression of PAR-4 in rat epididymisImmunohistochemical staining of rat epididymis paraffin-embedded sections using Anti-PAR4 (F2RL3) (extracellular) Antibody (APR-034), (1:100). Note that strong and specific staining is present in both the pseudostratified epithelium (blue arrow) and the smooth muscle cells of the blood vessels (red arrow). Hematoxilin is used as the counterstain.
- (C)HLRGQRWPFGEAA(S)R, corresponding to amino acid residues 136-150 of human PAR-4 (Accession Q96RI0).