Overview
Type: Synthetic peptide
Form: Lyophilized powder
PAR4/F2RL3 (extracellular) Blocking Peptide (#BLP-PR034) is the original antigen used for immunization during Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034) generation. The blocking peptide binds and ‘blocks’ Anti-PAR4/F2RL3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates:1,2. Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:200).
3,4. Anti-PAR4 (F2RL3) (extracellular) Antibody, preincubated with PAR4/F2RL3 (extracellular) Blocking Peptide (#BLP-PR034).
Western blot analysis of human prostate carcinoma PC3 (lanes 1 and 4), and Human LNCaP prostate carcinoma (lanes 2 and 5), and human T cell leukemia Jurkat (lanes 3 and 6) cell lines:1-3. Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:200).
4-6. Anti-PAR4 (F2RL3) (extracellular) Antibody, preincubated with PAR4/F2RL3 (extracellular) Blocking Peptide (#BLP-PR034).
Expression of PAR-4 in rat lungImmunohistochemical staining of rat lung paraffin-embedded sections using Anti-PAR4 (F2RL3) (extracellular) Antibody (APR-034), (1:100). Note that staining is present in the respiratory epithelium of the bronchiole (blue arrow) as well as in the smooth muscle cells of the muscularis mucosae (red arrow). Hematoxilin is used as the counterstain.
Expression of PAR-4 in rat epididymisImmunohistochemical staining of rat epididymis paraffin-embedded sections using Anti-PAR4 (F2RL3) (extracellular) Antibody (APR-034), (1:100). Note that strong and specific staining is present in both the pseudostratified epithelium (blue arrow) and the smooth muscle cells of the blood vessels (red arrow). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)HLRGQRWPFGEAA(S)R, corresponding to amino acid residues 136-150 of human PAR-4 (Accession Q96RI0).
Accession (Uniprot) Number Q96RI0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
