Plexin-A3 (extracellular) Blocking Peptide (#BLP-PR093) is the original antigen used for immunization during Anti-Plexin-A3 (extracellular) Antibody (#APR-093) generation. The blocking peptide binds and ‘blocks’ Anti-Plexin-A3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
- Expression of Plexin-A3 in mouse parietal cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Plexin-A3 (extracellular) Antibody (#APR-093), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. PLXNA3 staining (green) appears in pyramidal neurons (arrows). Cell nuclei are stained with DAPI (blue).
- (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3 (Accession P70208).