Anti-Plexin-A3 (extracellular) Antibody

PLXNA3, PLXN3, Semaphorin receptor SEX
    Cat #: APR-093
    Alternative Name PLXNA3, PLXN3, Semaphorin receptor SEX
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3 (Accession P70208). Extracellular, N-terminus.
    • Anti-Plexin-A3 (extracellular) Antibody
    Accession (Uniprot) Number P70208
    Gene ID 18846
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – identical; human – 12/14 amino acid residues identical.
    RRID AB_2756768.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, wb
    May also work in: ic*, ip*, lci*
    Western blot
    • Anti-Plexin-A3 (extracellular) Antibody
      Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
      1,2. Anti-Plexin-A3 (extracellular) Antibody (#APR-093), (1:200).
      3,4. Anti-Plexin-A3 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Plexin-A3 (extracellular) Antibody
      Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
      1. Anti-Plexin-A3 (extracellular) Antibody (#APR-093), (1:200).
      2. Anti-Plexin-A3 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Plexin-A3 (extracellular) Antibody
      Expression of Plexin-A3 in mouse parietal cortex
      Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Plexin-A3 (extracellular) Antibody (#APR-093), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. PLXNA3 staining (green) appears in pyramidal neurons (arrows). Cell nuclei are stained with DAPI (blue).
    Indirect flow cytometry
    • Anti-Plexin-A3 (extracellular) Antibody
      Cell surface detection of PLXNA3 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Plexin-A3 (extracellular) Antibody (#APR-093), (2.5 µg) + goat-anti-rabbit-FITC.
    References
    1. Zhang, L. et al. (2015) PLoS One 10, e0121513.
    2. Barton, R. et al. (2015) PLoS One 10, e0116368.
    Scientific background

    Plexins are type 1 transmembrane (TM) receptors acting as key regulatory proteins in a wide variety of developmental, regenerative and pathological processes. They are involved in guidance and motility of vascular, lymphatic vessel, and neuron growth, and in cancer metastasis.

    Plexin family includes 9 proteins: Plexin-A1-4, -B1-3, -C1 and -D1. Class A Plexins interact with the secreted class 3 Semaphorins, and require Neuropilins (nrps) as co-receptors.

    Plexin protein signaling results in cytosolic GTPase-activating 

    protein activity including direct interactions with Rho GTPases and transient/catalytic interactions with Ras GTPases1,2.

    Plexin structure consists of an extracellular semaphorin domain, three Plexin-Semaphorin-Integrin (PSI) domains, and three immunoglobulin, Plexin, and transcription factor (IPT) domains. In addition, a single-spanning transmembrane domain, and a cytosolic region (CYTO) homologous with Ras GTPase-activating proteins (GAPs)2. The transmembrane region of Plexin-A3 is characterized by poly-glycine usually used for close helix-helix packing and aromatic residues that distort helix conformation and association. Plexin-A family members only have 22 residues spanning the membrane1.

    Mutations in Plexins have been reported in melanoma, lung, breast, pancreatic, and prostate cancers, strongly suggesting that their signaling may play a role in cancer development. Plexins are widely expressed in the nervous system and in the developing cerebral cortex2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Plexin-A3 (extracellular) Antibody (#APR-093) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize PLXNA3 from rat, mouse, and human samples.

    For research purposes only, not for human use
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