Overview
Cat #: BLP-CP003
Type: Synthetic peptide
Form: Lyophilized powder
PMCA3 Blocking Peptide (#BLP-CP003) is the original antigen used for immunization during Anti-PMCA3 Antibody (#ACP-003) generation. The blocking peptide binds and ‘blocks’ Anti-PMCA3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-PMCA3 Antibody (#ACP-003), (1:200).
3,4. Anti-PMCA3 Antibody, preincubated with PMCA3 Blocking Peptide (#BLP-CP003). - Expression of PMCA3 in rat choroid plexusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PMCA3 Antibody (#ACP-003), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. PMCA3 immunoreactivity (green) appears in the outlines of the choroid plexus (arrows) located in the lateral ventricle of the brain. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
Accession (Uniprot) Number Q64568
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.