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Anti-PMCA3 Antibody

Plasma membrane Ca2+ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1

Cat #: ACP-003
Alternative Name Plasma membrane Ca2+ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)EFHPKPQ(N)QREVPH, corresponding to amino acid residues 10-23 of rat PMCA3 (Accession Q64568). Intracellular, N-terminus.
Accession (Uniprot) Number Q64568
Gene ID 29599
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse – 13/14 amino acid residues identical; human – 11/14 amino acid residues identical.
RRID AB_2756565.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    1,2. Anti-PMCA3 Antibody (#ACP-003), (1:200).
    3,4. Anti-PMCA3 Antibody, preincubated with PMCA3 Blocking Peptide (#BLP-CP003).
Immunohistochemistry
  • Expression of PMCA3 in rat choroid plexus
    Expression of PMCA3 in rat choroid plexus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PMCA3 Antibody (#ACP-003), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. PMCA3 immunoreactivity (green) appears in the outlines of the choroid plexus (arrows) located in the lateral ventricle of the brain. Cell nuclei are stained with DAPI (blue).
References
  1. Padányi, R. et al. (2016) Biochim. Biophys. Acta 1863, 1351.
  2. Monesterolo, N.E. et al. (2015) Biochim Biophys Acta 1848, 2813.
  3. Delgado-Coello, B. et al. (2014) Organogenesis 10, 333.
Scientific background

PMCAs (plasma membrane calcium ATPases) are P-type ATPases, a separate sub-group of Ca2 + pumps responsible for maintain intracellular Ca2+ homeostasis. PMCAs reduce Ca2 + in the cytosol by direct ejection, by controlling the formation of inositol-1,4,5-trisphosphate (IP3), and by decreasing Ca2 + release from the endoplasmic reticulum Ca2+ pool1,2.

Four PMAC proteins are expressed in mammals: PMCA1–4. Alternative splicing produces more than twenty variants that are dissimilar in their regulatory characteristics1,3. PMCA pumps have a C-terminus with a high affinity calmodulin-binding site that also acts as an auto-inhibitor. The C-terminal region also has phosphorylation sites as well as proteolytic sites for caspase-3 and calpain and several motifs that control trafficking of the pump1,2.

PMCA3 is usually present in excitable cells, in the brain and in hair cells of the inner ear or skeletal muscle. The variant is quickly activated and stays active for a long period of time1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-PMCA3 Antibody (#ACP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA3 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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