Presenilin-2 Blocking Peptide (#BLP-IP012) is the original antigen used for immunization during Anti-Presenilin-2 Antibody (#AIP-012) generation. The blocking peptide binds and ‘blocks’ Anti-Presenilin-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat heart membrane:
- Western blot analysis of mouse liver membrane:
- Western blot analysis of human liver carcinoma HepG2 (lanes 1 and 3) and rat pheochromocytoma PC12 (lanes 2 and 4) cell lysates:
- Expression of Presenilin-2 in mouse midbrainImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Presenilin-2 Antibody (#AIP-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Presenilin-2 immunoreactivity (green) appears in neurons along the midline (horizontal arrows) and away from midline (vertical arrows). B. Pre-incubation of the antibody with Presenilin-2 Blocking Peptide (BLP-IP012), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)DSRPGPEDGENTAQWR, corresponding to amino acid residues 33-48 of rat Presenilin-2 (Accession O88777).