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proBDNF Positive Control for Western Blot

Cat #: PCP-B257
Lyophilized Powder yes
Product Components: Recombinant human proBDNF in Laemmli Buffer
Form Lyophilized powder. Reconstituted proBDNF contains Laemmli Bufferx1
Amount 100 ng of proBDNF. Sufficient for two SDS-PAGE lanes.
Content Recombinant human BDNF in Laemmli Buffer.
Storage before reconstitution The product ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 20 µl double distilled water (DDW).
BDNF concentration after reconstitution 5 ng/µl.
Standard quality control of each lot Western blot analysis
Applications: wb
Positive control for Western blot
Western blot
  • Method of Use
    Dissolve the lyophilized powder by adding 20µl double distilled water (DDW). Heat the solubilized product to 100°C for 5 minutes. Load 10µl of the solution into an SDS-PAGE lane alongside the experimental samples. Run the gel and perform Western blot with an Anti-proBDNF antibody as per your usual protocol. For example, our own Anti-proBDNF Antibody (ANT-006).
  • Expected Result
    Single band detected at 26kD.
  • Western blot analysis using proBDNF Positive Control
    Western blot analysis using proBDNF Positive Control
    Lanes 1 and 2: 10µl (corresponding to 50ng) per lane of proBDNF Positive Control for Western Blot (#PCP-B257), was run in a SDS-PAGE and detected in a Western blot using Anti-proBDNF Antibody (#ANT-006), (1:200).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Last update: 13/10/2022

proBDNF Positive Control for Western Blot (#PCP-B257), is a recombinant, highly purified proBDNF protein in a ready-to-use single reagent format. This makes it a good positive control by loading it alongside the experimental lysates or samples in a SDS-PAGE. This product enables to check whether the primary and secondary antibodies are working as expected and also visualize a reference of the expected molecular weight of the target protein.

For research purposes only, not for human use
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