This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
This lyophilized product is shipped at room temperature. Please see its certificate of analysis for further storage instructions.
2,6. Recombinant mouse proBDNF protein (#B-240).
3,7. Recombinant human beta-NGF protein (#N-245).
4,8. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260).
Lanes 1-4: Anti-BDNF Antibody (#ANT-010), (1:200).
Lanes 5-8: Anti-BDNF Antibody, preincubated with the control peptide antigen.
Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.
All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.
BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system. Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its “rapid” neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.
Species reactivity key:
Expresssion of BDNF in mouse brain
Immunohistochemical staining of mouse brain sections using Anti-BDNF Antibody (#ANT-010). BDNF staining (purple) is expressed in astrocytes and co-localizes with GFAP (blue) but not with neurofilament-1 (NF-L), (green).Adapted from Fulmer, C.G. et al. (2014) J. Neurosci. 34, 8186. with permission of the Society for Neuroscience.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human brain-derived neurotrophic factor. Anti-BDNF Antibody (#ANT-010) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human, and mouse samples. The antibody is specific for BDNF; it does not crossreact with NGF, NT-3 or NT-4.
- Rat brain lysate (1:1000).
Hovens, I.B. et al. (2015) Am. J. Physiol. 309, R148.
- Mouse brain lysate (1:200).
Duarte-Neves, J. et al. (2015) Hum. Mol. Genet. 24, 5451.
- Human SH-SY5Y and rat primary cortical neuron lysates (1:1000).
Shin, M.K. et al. (2014) Neuropharmacology 77, 414.
- Mouse brain lysate (1:2000).
Shin, M.K. et al. (2014) Neurobiol. Aging. 35, 990.
- Rat lamina terminalis lysate (1:200).
Clayton, S.C. et al. (2014) Am. J. Physiol. 306, R908.
- Rat brain lysates (1:500).
Tian, X. et al. (2013) PLoS ONE 8, e69252.
- BDNF transfected striatal cells (1:50).
Del Toro, D. et al. (2006) J. Neurosci. 26, 12748.
- Demel, C. et al. (2011) J. Neuroinflammation. 8, 7.
- Poblete-Naredo, I. et al. (2011) Neurochem. Int. 59, 1133.
- España, J. et al. (2010) J. Neurosci. 30, 9402.
- Kong, Li. et al. (2010) Neurobiol. Dis. 38, 446.
- Taliaz, D. et al. (2010) Mol Psychiatry 15, 80.
- Lewitus, G.M. et al. (2008) Brain Behav. Immun. 22, 1108.
- Fiszman, M.L. et al. (2005) J. Neurosci. 25, 2024.
Antibodies for immuno-colocalization experiments
- Guinea pig Anti-BDNF Antibody (#AGP-021) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #ANT-010.
- Anti-proBDNF Antibody (#ANT-006)
- Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007)
- Anti-TrkB (extracellular) Antibody (#ANT-019)
- Recombinant human BDNF protein (#B-250)
- Recombinant human proBDNF protein (#B-257)
- Guinea pig Anti-proBDNF Antibody (#AGP-032)
- Anti-TrkB (extracellular)-ATTO-488 Antibody (#ANT-019-AG)
- Anti-TrkB (extracellular)-FITC Antibody (#ANT-019-F)