proNT-3 Blocking Peptide (#BLP-NT012) is the original antigen used for immunization during Anti-proNT-3 Antibody (#ANT-012) generation. The blocking peptide binds and ‘blocks’ Anti-proNT-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis using Anti-proNT-3 Antibody (#ANT-012) (1:600):1. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (200 ng).
2. Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (200 ng).
3. Native mouse NGF 2.5S protein (>95%) (#N-100), (200 ng).
4. Recombinant human beta-NGF protein (#N-245), (200 ng).
5. Recombinant human BDNF protein (#B-250), (200 ng).
6. Recombinant mouse proNGF protein (#N-250), (200 ng).
7. Recombinant mouse proBDNF protein (#B-240), (200 ng).
8. Recombinant proNT-3, (10 ng).
- Western blot analysis of mouse recombinant proNT-3:
- Expression of proNT-3 in rat brainImmunohistochemical staining of rat Islands of Calleja (IOC) using Anti-proNT-3 Antibody (#ANT-012). A. proNT-3 staining (green) overlaps neuronal nitric oxide synthase (nNOS red) while a cluster of cells covers a part of the island (DAPI blue counterstain) but not a cell-poor area (star). B. The distribution of proNT-3 is shown alone. Note that staining is more intense in the cell poor area demarcated by white line. C. The distribution of neuronal NOS is most intense in the cell-poor region.
- (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor) (Accession P20783).