Overview
Type: Synthetic peptide
Form: Lyophilized powder
proNT-3 Blocking Peptide (#BLP-NT012) is the original antigen used for immunization during Anti-proNT-3 Antibody (#ANT-012) generation. The blocking peptide binds and ‘blocks’ Anti-proNT-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis using Anti-proNT-3 Antibody (#ANT-012) (1:600):1. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (200 ng).
2. Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (200 ng).
3. Native mouse NGF 2.5S protein (>95%) (#N-100), (200 ng).
4. Recombinant human beta-NGF protein (#N-245), (200 ng).
5. Recombinant human BDNF protein (#B-250), (200 ng).
6. Recombinant mouse proNGF protein (#N-250), (200 ng).
7. Recombinant mouse proBDNF protein (#B-240), (200 ng).
8. Recombinant proNT-3, (10 ng).
Western blot analysis of mouse recombinant proNT-3:1,5. 10 ng proNT-3.
2,6. 50 ng proNT-3.
3,7. 100 ng proNT-3.
4,8. 200 ng proNT-3.
Lanes 1-4 Anti-proNT-3 Antibody (#ANT-012), (1:300).
Lanes 5-8 Anti-proNT-3 Antibody, preincubated with proNT-3 Blocking Peptide (#BLP-NT012).
Expression of proNT-3 in rat brainImmunohistochemical staining of rat Islands of Calleja (IOC) using Anti-proNT-3 Antibody (#ANT-012). A. proNT-3 staining (green) overlaps neuronal nitric oxide synthase (nNOS red) while a cluster of cells covers a part of the island (DAPI blue counterstain) but not a cell-poor area (star). B. The distribution of proNT-3 is shown alone. Note that staining is more intense in the cell poor area demarcated by white line. C. The distribution of neuronal NOS is most intense in the cell-poor region.
Properties
Sequence
- (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor) (Accession P20783).
Accession (Uniprot) Number P20783
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
