PSD-95 Blocking Peptide (#BLP-PZ009) is the original antigen used for immunization during Anti-PSD-95 Antibody (#APZ-009) generation. The blocking peptide binds and ‘blocks’ Anti-PSD-95 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
- Expression of PSD-95 in mouse parietal cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PSD-95 Antibody (#APZ-009), (1:400), followed by anti-rabbit-Cy3 antibody (red). Staining appears mainly in the pyramidal cell body (arrows). Nuclei were stained with DAPI (blue).
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- (C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95 (Accession P78352).