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Puromycin

Puromycin dihydrochloride, Stylomycin, P638
A Protein Synthesis Inhibitor and an Apoptosis Inducer
Cat #: P-540
Alternative Name Puromycin dihydrochloride, Stylomycin, P638
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Streptomyces alboniger.
    Source Natural
    MW: 544.4
    Purity: >99% (HPLC)
    Effective concentration 2-60 µM.
    Structure
    Chemical name 3'-[alpha-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride.
    Molecular formula C22H31Cl2N7O5 (C22H29N7O5·2HCl).
    CAS No.: 58-58-2.
    PubChem CID 443311
    Activity Puromycin is an antibiotic known to inhibit protein synthesis1, as well as an apoptosis inducer2. Puromycin can be used as a selection agent for mammalian cells, transformed to express puromycin-N-acetyl-transferase, at concentrations ranging between 0.5-2 µg/ml for suspension cell lines, and 2-6 µg/ml for adherent cell lines.
    References-Activity
    1. Davidoff, A.N. and Mendelow, B.V. (1992) Leuk. Res16, 1077.
    2. Gorski, J. et al. (1961) Arch. Biochem. Biophy95, 508.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Water. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Puromycin induces apoptosis in Jurkat cells.
      Alomone Labs Puromycin induces apoptosis in Jurkat cells.
      Cells were grown to 70% confluence. Then, 2 µM Puromycin (#P-540) or vehicle was added for 6 hours. At the end of the incubation, cell proteins were resolved by SDS-PAGE and probed with anti-cleaved Caspase 3 antibodies.
    References - Scientific background
    1. Vázquez, D. (1979) Inhibitors of Protein Biosynthesis, Springer-Verlag, Berlin.
    2. Yarmolinsky, M.B. and De La Haba,G.L. (1959) Proc. Natl. Acad. Sci. U. S. A. 45, 1721.
    3. Takeda, Y. et al. (1960) J. Biochem48, 169.
    4. Ferguson, J.J. (1962) Biochim. Biophys. Acta. 57, 616.
    5. Allen, D.W. and Zamecnik, P.C. (1962) Biochim. Biophys. Acta. 55, 865.
    6. Cundliffe, E. (1989) Annu. Rev. Microbiol43, 207.
    7. Constam, D.B. et al.  (1995) J. Biol. Chem270, 26931
    8. Schomburg, L. et al. (2000) Eur. J. Biochem267, 3198.
    Scientific background

    Puromycin is an aminoacyl nucleoside antibiotic produced by Streptomyces alboniger. It is a broad spectrum, non-specific inhibitor of protein synthesis in Gram-positive bacteria, protozoans, and mammalian cells, including tumor cells, and is widely used as a key compound in various cell-free systems directed to elucidate the mechanism of protein synthesis and the mode of action of other inhibitors of this process.1

    Puromycin is an analog for the 3' terminal of Tyr-tRNATyr which inhibits the enzyme peptidyl-transferase located in the 30s ribosomal subunits. Puromycin inhibits the enzyme by competition with aminoacyl-tRNA on the binding to the A' site or by attacking the peptidyl-tRNA in the P site of the enzyme. By this, the growing peptide chains on ribosomes are linked to the α-amino group of puromycin and interrupt the normal reaction of peptide bond formation.2-6

    Low levels of puromycin were found to act as an inhibitor of the soluble aminopeptidase (puromycin-sensitive aminopeptidase-PSA) in a variety of tissues. PSA inhibition by low puromycin concentrations that do not interfere with protein synthesis may directly lead to cell cycle arrest at the G2/M phase and induction of apoptosis.7,8

    Last update: 16/08/2020

    Puromycin (#P-540) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use

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    Scientific Background

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