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A23187

Calcimycin, Calcium ionophore A23187

Widely Used Ca2+ Ionophore

Cat #: A-600
Alternative Name Calcimycin, Calcium ionophore A23187
Lyophilized Powder yes
  • Bioassay Tested
    • Origin Streptomyces chartreusis.
    Source Natural
    MW: 523.6
    Purity: >98%
    Effective concentration 1-10 µM.
    Structure
    Chemical name 5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11- trimethy l-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7- dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid.
    Molecular formula C29H37N3O6.
    CAS No.: 52665-69-7
    Activity A23187 is a mobile-carrier Ca2+ ionophore and can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors1,2. Furthermore, A23187 induces, in a concentration- and time-dependent manner, Ca2+ triggered cell apoptosis3,4.
    References-Activity
    1. Berridge, M.J. (1993) Nature 361, 315.
    2. Scharff, O. et al. (1993) Physiol. Rev. 73, 547.
    3. Orrenius, S. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 552.
    4. Hajnoczky, G. et al. (2003) Biochem. Biophys. Res. Commun. 304, 445.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO, ethanol or methanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
    Protect from light.
    Our bioassay
    • Alomone Labs A23187 induces apoptosis in Jurkat cells.
      Alomone Labs A23187 induces apoptosis in Jurkat cells.
      2 µM A23187 (#A-600) or vehicle was added to the confluent cell culture for 6 hours. Apoptosis was probed by the detection of cleaved Caspase 3 using Caspase 3 specific antibodies in western blot analysis of cell lysates.
    References - Scientific background
    1. Berridge, M.J. (1993) Nature 361, 315.
    2. Scharff, O. et al. (1993) Physiol. Rev. 73, 547.
    3. Orrenius, S. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 552.
    4. Hajnoczky, G. et al. (2003) Biochem. Biophys. Res. Commun. 304, 445.
    5. Caron-Leslie, L.A. et al. (1991) Mol. Endocrinol. 5, 1169.
    6. Caron-Leslie, L.A. et al. (1994) FASEB J. 8, 639.
    7. Rzigalinski, B.A. et al. (1994) Biochim. Biophys. Acta. 1223, 219.
    8. Vrana, J.A. et al. (1998) Int. J. Oncol. 12, 927.
    9. Petersen, A. et al. (2000) Brain Res. 857, 20.
    10. Cheng, C.H. et al. (2002) FEBS Lett. 516, 191.
    11. Jansen, S. et al. (2003) Biol. Trace Elem. Res. 96, 133.
    Scientific background

    A23187 is a mobile-carrier Ca2+ ionophore (allows Ca2+ ions to cross cell membranes) originally isolated as an antibiotic from Streptomyces chartreusensis. It is used to increase intracellular Ca2+ levels and for in vitro studies related to intracellular Ca2+-mediated events. A23187 can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors.1,2

    Furthermore, A23187 induces, in a concentration- and time-dependent manner, Ca2+-triggered cell apoptosis.3,4 This property was exploited to clarify apoptotic events in different cell lines such as mouse lymphoma cell line (S49),5,6 histiocytic lymphoma U937,7 human myeloid leukemia cells (HL-60),8 cultured striatal neurons,9 renal tubular cells,10 and C6 glioma cells.11

    Lyophilized Powder
    Last update: 12/08/2021

    A23187 (#A-600) is a highly pure, natural, and biologically active compound.

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    For research purposes only, not for human use

    Applications

    Citations

    Citations
    Product citations
    1. Sosa, C.M. et al. (2015) Mol. Hum. Reprod. 21, 244.
    2. Paramo, B. et al. (2013) Int. J. Biochem. Cell Biol. 45, 2596.
    3. Suhaiman, L. et al. (2010) J. Biol. Chem. 285, 16302.

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